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magnetic positive selection  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec magnetic positive selection
    Magnetic Positive Selection, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/magnetic+positive+selection/CD3%CE%B5+Antibody%2C+anti-mouse%2C+REAfinity/pm41957474-222-13-23
    Average 99 stars, based on 47 article reviews
    magnetic positive selection - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Single Cell:

    Article Title: PD-1 blockade restores helper activity of tumor-infiltrating, exhausted PD-1 hi CD39 + CD4 T cells
    Article Snippet: .. CD4 + and CD8 + cells were enriched from tumor single-cell suspensions by magnetic positive selection (CD4 or CD8 MicroBeads, human, Miltenyi Biotec) using OctoMACS Separator and MS Columns. ..

    Article Title: Terminal T-cell exhaustion predicts tumor control independent of immunotherapy in ovarian cancer.
    Article Snippet: .. Cell purification and phenotypic assessment CD3+, CD4+ or CD8+ cells were enriched by magnetic positive selection (CD3 MicroBeads, CD8 Microbeads or CD4 Microbeads, Miltenyi Biotec) from thawed tumor single-cell suspensions. .. Cells were stained to assess viability using BD Horizon Fixable Viability Stain 700 (BD Biosciences, USA) or Fixable Viability Dye eFluor 506 (eBioscience, USA), in DPBS for 20 Minutes at 4°C.

    Selection:

    Article Title: PD-1 blockade restores helper activity of tumor-infiltrating, exhausted PD-1 hi CD39 + CD4 T cells
    Article Snippet: .. CD4 + and CD8 + cells were enriched from tumor single-cell suspensions by magnetic positive selection (CD4 or CD8 MicroBeads, human, Miltenyi Biotec) using OctoMACS Separator and MS Columns. ..

    Article Title: Combined MEK and JAK inhibition reduces osteopontin plasma level and bone marrow fibrosis in a myelofibrosis mouse model
    Article Snippet: .. Monocytes were then isolated from mononuclear 32 cells via magnetic positive selection (CD14 MicroBeads UltraPure, human, Miltenyi Biotec; 33 Auburn, CA, USA). .. Sample purity, always exceeding 95%, was confirmed by flow cytometry 34 with a PE-conjugated mouse anti-human CD14 antibody (Miltenyi Biotec) 35 36 In vitro treatment of human monocytes with Cobimetinib 37 Freshly isolated CD14+ monocytes were seeded at a density of 5x105 cells/ml and cultured 38 in IMDM (Euroclone, Milan, Italy) supplemented with 10% fetal bovine serum (FBS, Hyclone, 39 Cytiva, Marlborough, MA, USA) and GM-CSF (20 ng/mL; Miltenyi Biotec; Auburn, CA, USA).

    Article Title: PU.1 Shapes Host Epigenetic Responses to Mycobacterium tuberculosis and Represents a Target for Host Directed Therapy
    Article Snippet: .. To prepare human monocyte-derived macrophages (hMDM), CD14 + monocytes were isolated directly from buffy coat (Etablissement Français du Sang, convention N° 21RB2025-028-R) using magnetic positive selection (Miltenyi Biotec, #130-114-976), following the manufacturer’s instructions. .. The purified monocytes were differentiated into macrophages by culturing them in complete RPMI-1640 medium supplemented with 10% FCS (Sigma-Aldrich, #F7524), and 100 ng/ml recombinant human M-CSF (R&D systems, #216-MC-500) for 6 days.

    Article Title: Terminal T-cell exhaustion predicts tumor control independent of immunotherapy in ovarian cancer.
    Article Snippet: .. Cell purification and phenotypic assessment CD3+, CD4+ or CD8+ cells were enriched by magnetic positive selection (CD3 MicroBeads, CD8 Microbeads or CD4 Microbeads, Miltenyi Biotec) from thawed tumor single-cell suspensions. .. Cells were stained to assess viability using BD Horizon Fixable Viability Stain 700 (BD Biosciences, USA) or Fixable Viability Dye eFluor 506 (eBioscience, USA), in DPBS for 20 Minutes at 4°C.

    Article Title: Developmental Differences Between Neonatal and Adult Human Erythropoiesis
    Article Snippet: .. CD34 + cells were purified from CB or non-mobilized PB leukoreduction filters by magnetic positive selection (Miltenyi Biotec). .. Erythroid differentiation of isolated CD34 + cells ( Table 1 ) was monitored as a function of surface marker expression on a BD LSR Fortessa (Becton Dickinson) and data subsequently analyzed using FCS express 6 software (De Novo, Inc) as previously described[ 18 , 21 ].

    Article Title: BRAF-specific TCRs and uses thereof
    Article Snippet: Antigen-specific T cells were further enriched by sorting for Vβ3.1 positive, CD4+ cells by staining with anti-Vβ 3.1 (Thermo Scientific, cat. no. TCR2740), expanded, and cryopreserved at day 13 or 14 after expansion. .. Antigen-Presenting Cells Autologous B cells were isolated from fresh or thawed PBMC using magnetic beads coated with antibodies recognizing CD19 (Miltenyi, cat. no. 130-050-301) and magnetic positive selection according to the manufacturer's instructions (Miltenyi, cat. no. 130-042-401). .. Antigen-Presenting Cells Autologous B cells were isolated from fresh or thawed PBMC using magnetic beads coated with antibodies recognizing CD19 (Miltenyi, cat. no. 130-050-301) and magnetic positive selection according to the manufacturer's instructions (Miltenyi, cat. no. 130-042-401).

    Article Title: The FcγRIIa-Syk Axis Controls Human Dendritic Cell Activation and T Cell Response Induced by Infliximab Aggregates.
    Article Snippet: .. As previously described (9), monocytes were isolated from the mononuclear fraction by magnetic positive selection with MidiMACS Separator separation columns and antiCD14 Abs coated on magnetic beads (Miltenyi Biotec, Bergish Gladbach, Germany). .. Monocytes were cultured at 1 3 106 cells/ml in the presence of GM-CSF (550 U/ml) and IL-4 (550 U/ml) (Miltenyi Biotec) in RPMI 1640 25 mM HEPES GlutaMAX supplemented with 10% FCS, antibiotics, and 1% pyruvate at 37 ̊C in humidified air containing 5% CO2.

    Article Title: Thymopoiesis, Alterations in Dendritic Cells and Tregs, and Reduced T Cell Activation in Successful Extracorporeal Photopheresis Treatment of GVHD.
    Article Snippet: .. Patients’ CD3+ T cells were purified by magnetic positive selection (Miltenyi Biotec), followed by RNA isolation with the RNeasy Micro Kit (Qiagen). cDNAwas synthesized from 10 ng of total RNA using SuperScriptTM VILOTM cDNA Synthesis Kit (ThermoFisher). ..

    Isolation:

    Article Title: Combined MEK and JAK inhibition reduces osteopontin plasma level and bone marrow fibrosis in a myelofibrosis mouse model
    Article Snippet: .. Monocytes were then isolated from mononuclear 32 cells via magnetic positive selection (CD14 MicroBeads UltraPure, human, Miltenyi Biotec; 33 Auburn, CA, USA). .. Sample purity, always exceeding 95%, was confirmed by flow cytometry 34 with a PE-conjugated mouse anti-human CD14 antibody (Miltenyi Biotec) 35 36 In vitro treatment of human monocytes with Cobimetinib 37 Freshly isolated CD14+ monocytes were seeded at a density of 5x105 cells/ml and cultured 38 in IMDM (Euroclone, Milan, Italy) supplemented with 10% fetal bovine serum (FBS, Hyclone, 39 Cytiva, Marlborough, MA, USA) and GM-CSF (20 ng/mL; Miltenyi Biotec; Auburn, CA, USA).

    Article Title: PU.1 Shapes Host Epigenetic Responses to Mycobacterium tuberculosis and Represents a Target for Host Directed Therapy
    Article Snippet: .. To prepare human monocyte-derived macrophages (hMDM), CD14 + monocytes were isolated directly from buffy coat (Etablissement Français du Sang, convention N° 21RB2025-028-R) using magnetic positive selection (Miltenyi Biotec, #130-114-976), following the manufacturer’s instructions. .. The purified monocytes were differentiated into macrophages by culturing them in complete RPMI-1640 medium supplemented with 10% FCS (Sigma-Aldrich, #F7524), and 100 ng/ml recombinant human M-CSF (R&D systems, #216-MC-500) for 6 days.

    Article Title: BRAF-specific TCRs and uses thereof
    Article Snippet: Antigen-specific T cells were further enriched by sorting for Vβ3.1 positive, CD4+ cells by staining with anti-Vβ 3.1 (Thermo Scientific, cat. no. TCR2740), expanded, and cryopreserved at day 13 or 14 after expansion. .. Antigen-Presenting Cells Autologous B cells were isolated from fresh or thawed PBMC using magnetic beads coated with antibodies recognizing CD19 (Miltenyi, cat. no. 130-050-301) and magnetic positive selection according to the manufacturer's instructions (Miltenyi, cat. no. 130-042-401). .. Antigen-Presenting Cells Autologous B cells were isolated from fresh or thawed PBMC using magnetic beads coated with antibodies recognizing CD19 (Miltenyi, cat. no. 130-050-301) and magnetic positive selection according to the manufacturer's instructions (Miltenyi, cat. no. 130-042-401).

    Article Title: The FcγRIIa-Syk Axis Controls Human Dendritic Cell Activation and T Cell Response Induced by Infliximab Aggregates.
    Article Snippet: .. As previously described (9), monocytes were isolated from the mononuclear fraction by magnetic positive selection with MidiMACS Separator separation columns and antiCD14 Abs coated on magnetic beads (Miltenyi Biotec, Bergish Gladbach, Germany). .. Monocytes were cultured at 1 3 106 cells/ml in the presence of GM-CSF (550 U/ml) and IL-4 (550 U/ml) (Miltenyi Biotec) in RPMI 1640 25 mM HEPES GlutaMAX supplemented with 10% FCS, antibiotics, and 1% pyruvate at 37 ̊C in humidified air containing 5% CO2.

    Article Title: Thymopoiesis, Alterations in Dendritic Cells and Tregs, and Reduced T Cell Activation in Successful Extracorporeal Photopheresis Treatment of GVHD.
    Article Snippet: .. Patients’ CD3+ T cells were purified by magnetic positive selection (Miltenyi Biotec), followed by RNA isolation with the RNeasy Micro Kit (Qiagen). cDNAwas synthesized from 10 ng of total RNA using SuperScriptTM VILOTM cDNA Synthesis Kit (ThermoFisher). ..

    Purification:

    Article Title: Terminal T-cell exhaustion predicts tumor control independent of immunotherapy in ovarian cancer.
    Article Snippet: .. Cell purification and phenotypic assessment CD3+, CD4+ or CD8+ cells were enriched by magnetic positive selection (CD3 MicroBeads, CD8 Microbeads or CD4 Microbeads, Miltenyi Biotec) from thawed tumor single-cell suspensions. .. Cells were stained to assess viability using BD Horizon Fixable Viability Stain 700 (BD Biosciences, USA) or Fixable Viability Dye eFluor 506 (eBioscience, USA), in DPBS for 20 Minutes at 4°C.

    Article Title: Developmental Differences Between Neonatal and Adult Human Erythropoiesis
    Article Snippet: .. CD34 + cells were purified from CB or non-mobilized PB leukoreduction filters by magnetic positive selection (Miltenyi Biotec). .. Erythroid differentiation of isolated CD34 + cells ( Table 1 ) was monitored as a function of surface marker expression on a BD LSR Fortessa (Becton Dickinson) and data subsequently analyzed using FCS express 6 software (De Novo, Inc) as previously described[ 18 , 21 ].

    Article Title: Thymopoiesis, Alterations in Dendritic Cells and Tregs, and Reduced T Cell Activation in Successful Extracorporeal Photopheresis Treatment of GVHD.
    Article Snippet: .. Patients’ CD3+ T cells were purified by magnetic positive selection (Miltenyi Biotec), followed by RNA isolation with the RNeasy Micro Kit (Qiagen). cDNAwas synthesized from 10 ng of total RNA using SuperScriptTM VILOTM cDNA Synthesis Kit (ThermoFisher). ..

    Magnetic Beads:

    Article Title: BRAF-specific TCRs and uses thereof
    Article Snippet: Antigen-specific T cells were further enriched by sorting for Vβ3.1 positive, CD4+ cells by staining with anti-Vβ 3.1 (Thermo Scientific, cat. no. TCR2740), expanded, and cryopreserved at day 13 or 14 after expansion. .. Antigen-Presenting Cells Autologous B cells were isolated from fresh or thawed PBMC using magnetic beads coated with antibodies recognizing CD19 (Miltenyi, cat. no. 130-050-301) and magnetic positive selection according to the manufacturer's instructions (Miltenyi, cat. no. 130-042-401). .. Antigen-Presenting Cells Autologous B cells were isolated from fresh or thawed PBMC using magnetic beads coated with antibodies recognizing CD19 (Miltenyi, cat. no. 130-050-301) and magnetic positive selection according to the manufacturer's instructions (Miltenyi, cat. no. 130-042-401).

    Article Title: The FcγRIIa-Syk Axis Controls Human Dendritic Cell Activation and T Cell Response Induced by Infliximab Aggregates.
    Article Snippet: .. As previously described (9), monocytes were isolated from the mononuclear fraction by magnetic positive selection with MidiMACS Separator separation columns and antiCD14 Abs coated on magnetic beads (Miltenyi Biotec, Bergish Gladbach, Germany). .. Monocytes were cultured at 1 3 106 cells/ml in the presence of GM-CSF (550 U/ml) and IL-4 (550 U/ml) (Miltenyi Biotec) in RPMI 1640 25 mM HEPES GlutaMAX supplemented with 10% FCS, antibiotics, and 1% pyruvate at 37 ̊C in humidified air containing 5% CO2.

    Synthesized:

    Article Title: Thymopoiesis, Alterations in Dendritic Cells and Tregs, and Reduced T Cell Activation in Successful Extracorporeal Photopheresis Treatment of GVHD.
    Article Snippet: .. Patients’ CD3+ T cells were purified by magnetic positive selection (Miltenyi Biotec), followed by RNA isolation with the RNeasy Micro Kit (Qiagen). cDNAwas synthesized from 10 ng of total RNA using SuperScriptTM VILOTM cDNA Synthesis Kit (ThermoFisher). ..

    cDNA Synthesis:

    Article Title: Thymopoiesis, Alterations in Dendritic Cells and Tregs, and Reduced T Cell Activation in Successful Extracorporeal Photopheresis Treatment of GVHD.
    Article Snippet: .. Patients’ CD3+ T cells were purified by magnetic positive selection (Miltenyi Biotec), followed by RNA isolation with the RNeasy Micro Kit (Qiagen). cDNAwas synthesized from 10 ng of total RNA using SuperScriptTM VILOTM cDNA Synthesis Kit (ThermoFisher). ..



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